Journal: Saudi Journal of Biological Sciences

Article Title: Endophytic fungi of Tradescantia pallida mediated targeting of Multi-Drug resistant human pathogens

doi: 10.1016/j.sjbs.2024.103937

Figure Lengend Snippet: Anti-Multiple Drug Resistant (MDR) bacterial activity of F.oxysporum (TPL11) against (A) MRSA ATCC 43300, (B) MRSA ATCC 700699 and (C) VRE ATCC 51299; N.sphaerica (TPL14) against (A’) MRSA ATCC 43300, (B’) MRSA ATCC 700699 and (C’) VRE ATCC 51299 by disc diffusion method at different concentrations (25, 50, 75, 100 µg/mL) after 16–18 hrs incubation.

Article Snippet: The resistant bacterial pathogens utilized were Methicillin-resistant Staphylococcus aureus -MRSA (ATCC 43300; 700699), Staphylococcus aureus (ATCC 25923), S.aureus (MTCC 3160), Vancomycin-resistant Enterococci- VRE (ATCC 51299) and Enterococcus fecalis .

Techniques: Activity Assay, Diffusion-based Assay, Incubation

Journal: Saudi Journal of Biological Sciences

Article Title: Endophytic fungi of Tradescantia pallida mediated targeting of Multi-Drug resistant human pathogens

doi: 10.1016/j.sjbs.2024.103937

Figure Lengend Snippet: Minimum Bacteriostatic and Bactericidal Concentration of ethyl acetate extract.

Article Snippet: The resistant bacterial pathogens utilized were Methicillin-resistant Staphylococcus aureus -MRSA (ATCC 43300; 700699), Staphylococcus aureus (ATCC 25923), S.aureus (MTCC 3160), Vancomycin-resistant Enterococci- VRE (ATCC 51299) and Enterococcus fecalis .

Techniques: Concentration Assay

Journal: Saudi Journal of Biological Sciences

Article Title: Endophytic fungi of Tradescantia pallida mediated targeting of Multi-Drug resistant human pathogens

doi: 10.1016/j.sjbs.2024.103937

Figure Lengend Snippet: Synergistic antibacterial effect of ethyl acetate fungal crude extract of F.oxysporum and N.sphaerica with conventional antibiotics against human pathogens.

Article Snippet: The resistant bacterial pathogens utilized were Methicillin-resistant Staphylococcus aureus -MRSA (ATCC 43300; 700699), Staphylococcus aureus (ATCC 25923), S.aureus (MTCC 3160), Vancomycin-resistant Enterococci- VRE (ATCC 51299) and Enterococcus fecalis .

Techniques:

Journal: PLoS ONE

Article Title: Antimicrobial activities of Bacillus velezensis strains isolated from stingless bee products against methicillin-resistant Staphylococcus aureus

doi: 10.1371/journal.pone.0251514

Figure Lengend Snippet: Antibacterial activities of B . velezensis strains against several pathogenic bacteria.

Article Snippet: All the strains of B . velezensis were able to inhibit MRSA strains (MSSA ATCC 12228, MRSA ATCC 700699, MRSA ATCC 25922, MRSA ATCC 33741 and MRSA ATCC 33742).

Techniques: Bacteria, Inhibition

Journal: PLoS ONE

Article Title: Antimicrobial activities of Bacillus velezensis strains isolated from stingless bee products against methicillin-resistant Staphylococcus aureus

doi: 10.1371/journal.pone.0251514

Figure Lengend Snippet: (a) The determination of MIC value. Growth of MRSA ATCC 33742 in nutrient broth inoculated with different dilutions of CFS from (i) strain PD9 and (ii) strain PD9 + MRSA ATCC 33742, after 16 h incubation. Un-inoculated nutrient broth serves as negative control. Reported values are mean ± standard deviation (error bar). (b) The determination of MBC value. Bactericidal activity of strain PD9 CFS from dilution factors of 2 1 to 2 3 on MRSA ATCC 33742 observed on mannitol salt agar after 24 h incubation. MIC = minimum inhibitory concentration, MBC = minimum bactericidal concentration and CFS = cell-free supernatant.

Article Snippet: All the strains of B . velezensis were able to inhibit MRSA strains (MSSA ATCC 12228, MRSA ATCC 700699, MRSA ATCC 25922, MRSA ATCC 33741 and MRSA ATCC 33742).

Techniques: Incubation, Negative Control, Standard Deviation, Activity Assay, Concentration Assay

Journal: PLoS ONE

Article Title: Antimicrobial activities of Bacillus velezensis strains isolated from stingless bee products against methicillin-resistant Staphylococcus aureus

doi: 10.1371/journal.pone.0251514

Figure Lengend Snippet: The inhibitory effect of strain PD9 at the final cell density of : 0 cfu/ml (control), : 10 3 cfu/ml, : 10 4 cfu/ml, and :10 5 cfu/ml on the growth of MRSA ATCC 33742. Reported values are mean ± standard deviation (error bar).

Article Snippet: All the strains of B . velezensis were able to inhibit MRSA strains (MSSA ATCC 12228, MRSA ATCC 700699, MRSA ATCC 25922, MRSA ATCC 33741 and MRSA ATCC 33742).

Techniques: Control, Standard Deviation

Journal: PLoS ONE

Article Title: Antimicrobial activities of Bacillus velezensis strains isolated from stingless bee products against methicillin-resistant Staphylococcus aureus

doi: 10.1371/journal.pone.0251514

Figure Lengend Snippet: (a) Thermal stability of strain PD9 CFS on MRSA ATCC 33742 lawn cell plate after the heat treatment. Strain PD9 CFS was treated at 40 to 100°C for 30 min. The inhibitory activity was tested using agar well diffusion assay. CFS at room temperature served as a control. (b) pH stability of strain PD9 CFS on MRSA ATCC 33742 lawn cell plate after 30 min of pH treatment. The buffers used for the treatments were sodium acetate (pH4-6), sodium phosphate (pH 6–8), Tris-HCI (pH 8–9), glycine-OH (pH 9–11) and sodium hydrogen phosphate (pH 11–12). The buffer alone served as a negative control. (c) Enzyme stability of strain PD9 CFS on MRSA ATCC 33742 lawn cell plate after strain PD9 CFS was treated with proteinase K for 0.5 to 2.0 h at 37°C ( ) and 55°C ( ). CFS without proteinase K treatment served as a control. Reported values are mean ± standard deviation (error bar); CFS = cell-free supernatant.

Article Snippet: All the strains of B . velezensis were able to inhibit MRSA strains (MSSA ATCC 12228, MRSA ATCC 700699, MRSA ATCC 25922, MRSA ATCC 33741 and MRSA ATCC 33742).

Techniques: Activity Assay, Diffusion-based Assay, Control, Negative Control, Standard Deviation

Journal: PLoS ONE

Article Title: Antimicrobial activities of Bacillus velezensis strains isolated from stingless bee products against methicillin-resistant Staphylococcus aureus

doi: 10.1371/journal.pone.0251514

Figure Lengend Snippet: Effect of surfactants and metal ions on inhibitory activities of strain PD9 CFS on MRSA ATCC 33742.

Article Snippet: All the strains of B . velezensis were able to inhibit MRSA strains (MSSA ATCC 12228, MRSA ATCC 700699, MRSA ATCC 25922, MRSA ATCC 33741 and MRSA ATCC 33742).

Techniques: Inhibition, Control

Journal: PLoS ONE

Article Title: Antimicrobial activities of Bacillus velezensis strains isolated from stingless bee products against methicillin-resistant Staphylococcus aureus

doi: 10.1371/journal.pone.0251514

Figure Lengend Snippet: (A) Antimicrobial activity of the 1-butanol extract observed (a) before and (b) after evaporation on MRSA ATCC 33742 lawn cell plate. The antimicrobial compound from the 24-h cell culture of strain PD9 was extracted using 1-butanol as the organic solvent. (B) Antimicrobial activity of the eluted fractions from different ratios of DCM: MeOH observed on MRSA ATCC 33742 lawn cell plate. (a) Active fractions from eluent with ratio DCM (3):(1) MeOH. (b) Active fractions from eluent with ratio DCM (5):(1) MeOH. (c) Active fractions from eluent with ratio DCM (7):(1) MeOH. (d) Active fractions from eluent with ratio DCM (10):(1) MeOH.

Article Snippet: All the strains of B . velezensis were able to inhibit MRSA strains (MSSA ATCC 12228, MRSA ATCC 700699, MRSA ATCC 25922, MRSA ATCC 33741 and MRSA ATCC 33742).

Techniques: Activity Assay, Evaporation, Cell Culture, Solvent

Journal: PLoS ONE

Article Title: Antimicrobial activities of Bacillus velezensis strains isolated from stingless bee products against methicillin-resistant Staphylococcus aureus

doi: 10.1371/journal.pone.0251514

Figure Lengend Snippet: A) Tricine–SDS-PAGE analysis and direct detection of antimicrobial activity of the antimicrobial peptide using zymogram. The single band of purified peptide observed on SDS-PAGE with a halo zone observed against MRSA ATCC 33742 on the zymogram after 24 h of incubation. Vertical black lines in between the AMP and zymogram represents different gels ( and Figs) merged together to show the direct inhibitory activity of AMP against MRSA ATCC 33742. Marker: Unstained low range protein ladder. AMP: Antimicrobial peptide. (B) HPLC analysis of the antimicrobial peptide. A single peak was observed on the chromatogram indicating the purity of the silica gel purified antimicrobial peptide indicating successful purification of the antimicrobial peptide.

Article Snippet: All the strains of B . velezensis were able to inhibit MRSA strains (MSSA ATCC 12228, MRSA ATCC 700699, MRSA ATCC 25922, MRSA ATCC 33741 and MRSA ATCC 33742).

Techniques: SDS Page, Activity Assay, Purification, Incubation, Marker

Journal: International Journal of Molecular Sciences

Article Title: Current State of Knowledge Regarding WHO High Priority Pathogens—Resistance Mechanisms and Proposed Solutions through Candidates Such as Essential Oils: A Systematic Review

doi: 10.3390/ijms24119727

Figure Lengend Snippet: Studies assessing the antimicrobial activity of essential oils against methicillin resistant, vancomycin-intermediate and -resistant S. aureus (a non-exhaustive list).

Article Snippet: Demirci F et al., 2018 , MRSA ATCC 700699 S. aureus ATCC BAA-1026 S. epidermidis ATCC 14990 S. pyogenes ATCC 19615 S. pneumoniae ATCC 10015 P. aeruginosa ATCC 10145 H. influenzae ATCC 49247 M. catarrhalis ATCC 23245 , Thymus sipyleus Boiss. , Agar diffusion Broth microdilution Vapour diffusion , The best antibacterial effect for thyme scented lemon was noticed against S. aureus , S. pyogenes and M. catarrhalis (MIC = 12–13 μg/mL). MRSA MIC = 310 μg/mL and P. aeruginosa MIC = 1250 μg/mL (lowest inhibitory effect). The anti-inflammatory effect was 12.1 ± 1.8% in 100 μg/mL, both effects being required for efficient inhalations in the treatment of rhinosinusitis. , [ ] .

Techniques: Activity Assay, Diffusion-based Assay, Inhibition, Cytotoxicity Assay, Electron Microscopy, In Vitro, Dilution Assay, Preserving, Quantitative Proteomics, Nucleic Acid Electrophoresis, Membrane, Confocal Laser Scanning Microscopy, Concentration Assay, Titration, Bacteria, Microscopy, Transmission Assay, Microdilution Assay, Produced, Modification, Clinical Proteomics, Blocking Assay, Staining, Cell Culture, Fourier Transform Infrared Spectroscopy, Spectroscopy, Reverse Transcription, Real-time Polymerase Chain Reaction, Crystal Violet Assay, Expressing, Flow Cytometry, In Vivo, Liposomes, Time-Kill Assay, Formulation, MTT Assay, Incubation, Thin Layer Chromatography, Bioassay, Antibiofilm Assay, Resazurin Assay, Biofilm Production Assay, Control, Infection, Cream, Antioxidant Activity Assay, Permeability, Virus, Extraction, Isolation

Journal: BioMed Research International

Article Title: In Vitro Inhibitory and Cytotoxic Activity of MFM 501, a Novel Codonopsinine Derivative, against Methicillin-Resistant Staphylococcus aureus Clinical Isolates

doi: 10.1155/2015/823829

Figure Lengend Snippet: MIC, MBC, and MBC/MIC values for MFM 501 against additional S. aureus isolates.

Article Snippet: Forty-three MRSA and 11 MSSA clinical isolates from three local hospitals with additional three American Type Culture Collection (ATCC) MRSA reference strains (ATCC 33591, ATCC 700699, and BAA-1556) and two MSSA ATCC reference strains (ATCC 25923 and ATCC 35556) were used in this experiment (as listed in ).

Techniques: